mouse tslp duoset elisa kit  (R&D Systems)

Journal: Nature Communications

Article Title: Context-dependent function of TSLP and IL-1β in skin allergic sensitization and atopic march

doi: 10.1038/s41467-022-32196-1

Figure Lengend Snippet: a RNAscope in situ hybridization for IL-1β mRNA in non-treated (NT), LMP_30μm and LMP_91 μm ears at 48 h after the microporation. Dashed lines indicate the dermal/epidermal junction. b ELISA measurement of IL-1β protein levels in ears at 48 h after LMP_30 μm and 91 μm ( n = 5, 12, 6 mice). c Experimental protocol. HDM with or without IL-1β was applied on LMP_30μm ears (e.c. HDM ± IL-1β), at day (D) 0, D3, D7 and D10. Mice were intranasally (i.n.) challenged with HDM every day from D9 to D12 to induce allergic asthma, and analysed at D13. d ELISA measurement of TSLP protein levels in 30 μm-LMP ears co-administrated with recombinant IL-1β or PBS ( n = 4 mice). e Hematoxylin & eosin (H&E) staining and immunohistochemistry (IHC) staining for MBP or MCPT8 on ear sections. Black arrows point to one of the positive signals. Scale bar = 50 μm for all pictures. f Comparison of CXCR5 + PD1 + Tfh cells, GL7 + Fas + GC B cells, IgE + B and IgG1 + B cells in EDLNs ( n = 4, 5, 3, 3, 3, 4 mice). g Serum levels of HDM-specific IgG1 and IgE in HDM-treated mice ( n = 5, 5, 6, 4 mice). Graphs in b , d , f show mean ± SEM. Two-sided Student’s t -test. Graphs in g show median. Two-sided Mann–Whitney rank sum test. All data are representative of two independent experiments with similar results. Source data are provided as a Source Data file.

Article Snippet: TSLP and IL-1β levels in skin extracts were determined using the DuoSet ELISA Development Kits (R&D Systems, Minneapolis, Minn, Cat No. DY555 for TSLP, Cat No. DY401 for IL-1β).

Techniques: RNAscope, In Situ Hybridization, Enzyme-linked Immunosorbent Assay, Recombinant, Staining, Immunohistochemistry, Comparison, MANN-WHITNEY

Journal: Nature Communications

Article Title: Context-dependent function of TSLP and IL-1β in skin allergic sensitization and atopic march

doi: 10.1038/s41467-022-32196-1

Figure Lengend Snippet: a Experimental protocol. Wildtype Balb/c mice were intraperitoneally (i.p.) injected with PBS, NIMP-R14 or anti-Ly6G antibody (Ab) at day (D) -1 and D2. Mice were d.c. sensitized with HDM on LMP_91μm ears at D0 and D3 or non-sensitized. All mice were intranasally (i.n.) challenged with HDM from D10 to D13 and analysed at D14. b Total cell number and differential cell counting in BAL fluid ( n = 4, 4, 4, 3 mice). c Relative mRNA levels of genes in BAL cells ( n = 4, 4, 3, 4 mice). d Lung sections were stained with hematoxylin-eosin (H&E) for histological analyses or Periodic Acid Schiff (PAS) for goblet cell hyperplasia analyses. B: bronchiole. V: blood vessel. Bar = 250 μm for all pictures. e Serum level of HDM-specific IgE measured by ELISA ( n = 3 mice). Graphs in b , c show mean ± SEM, One-way ANOVA test. Graph in e marks median, two-sided Mann–Whitney rank sum test. All data are representative of two independent experiments with similar result. Source data are provided as a Source Data file.

Article Snippet: TSLP and IL-1β levels in skin extracts were determined using the DuoSet ELISA Development Kits (R&D Systems, Minneapolis, Minn, Cat No. DY555 for TSLP, Cat No. DY401 for IL-1β).

Techniques: Injection, Cell Counting, Staining, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Nature Communications

Article Title: Context-dependent function of TSLP and IL-1β in skin allergic sensitization and atopic march

doi: 10.1038/s41467-022-32196-1

Figure Lengend Snippet: a Experimental protocol. Wildtype Balb/c mice were intraperitoneally (i.p.) injected with PBS or NIMP-R14 antibody at day (D) -1 and D2. Mice were d.c. sensitized with HDM ± IL-1β on LMP_91μm ears at D0 and D3. All mice were intranasally (i.n.) challenged with HDM from D9 to D12 and analysed at D13. b Total cell number and differential cell counting in BAL fluid ( n = 5, 4, 5 mice). One-way ANOVA test. c Relative RNA levels of genes in BAL cells ( n = 5, 4, 4 mice). One-way ANOVA test. d Lung sections were stained with hematoxylin-eosin (H&E) or Periodic Acid Schiff (PAS). B: bronchiole. V: blood vessel. Bar = 250 μm for all pictures. e Lung resistance (R L ) at the baseline (aerosol of PBS) and in response to aerosolized methacholine (Mch; 50 mg/ml), measured by FlexiVent system ( n = 4, 5, 4, 5 mice). Two-sided Student’s t test. f Serum level of HDM-specific IgE and IgG1 measured by ELISA ( n = 5, 4, 5 mice). Two-sided Mann–Whitney rank sum test. NS, non-sensitized. Data are representative of 2 ( b – d , f ) or 1 ( e ) independent experiments with similar results. Source data are provided as a Source Data file.

Article Snippet: TSLP and IL-1β levels in skin extracts were determined using the DuoSet ELISA Development Kits (R&D Systems, Minneapolis, Minn, Cat No. DY555 for TSLP, Cat No. DY401 for IL-1β).

Techniques: Injection, Cell Counting, Staining, Aerosol, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Impressic Acid Ameliorates Atopic Dermatitis-Like Skin Lesions by Inhibiting ERK1/2-Mediated Phosphorylation of NF-κB and STAT1

doi: 10.3390/ijms22052334

Figure Lengend Snippet: Inhibitory effect of IPA on the DNCB-induced increased serum concentration and mRNA level of thymic stromal lymphopoietin (TSLP) in BALB/C mice. ( A ) Serum TSLP concentrations were assessed by ELISA. ( B ) TSLP mRNA level was assessed by real-time PCR. Results are means ± SD (n = 6). # p < 0.05, versus the control group; * p < 0.05, versus the DNCB-treated group.

Article Snippet: The serum concentrations of IgE and TSLP were assessed by sandwich ELISA using an OptEIA Mouse IgE Kit (BD Biosciences, San Diego, CA, USA) and a DuoSet Mouse TSLP Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ protocols.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Control

Journal: International Journal of Molecular Sciences

Article Title: Impressic Acid Ameliorates Atopic Dermatitis-Like Skin Lesions by Inhibiting ERK1/2-Mediated Phosphorylation of NF-κB and STAT1

doi: 10.3390/ijms22052334

Figure Lengend Snippet: Inhibitory effect of IPA on the mRNA levels of TSLP, TARC, and MDC in TNF-α/IFN-γ-stimulated HaCaT cells. ( A ) Cells were treated with various concentrations of IPA for 24 h. Cell viability was assessed by MTT reduction assay. ( B ) Cell cytotoxicity was determined by LDH release assay. ( C – E ) Cells were treated with IPA (1, 5 or 10 μM) for 1 h, and then stimulated with TNF-α/IFN-γ (each 10 ng/mL) for 6 h, and the mRNA levels of TSLP, TARC, and MDC were determined by real-time PCR. Results are means ± SD (n = 3). # p < 0.01, versus the control group. * p < 0.01, versus the TNF-α/IFN-γ-treated group.

Article Snippet: The serum concentrations of IgE and TSLP were assessed by sandwich ELISA using an OptEIA Mouse IgE Kit (BD Biosciences, San Diego, CA, USA) and a DuoSet Mouse TSLP Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ protocols.

Techniques: MTT Reduction Assay, Lactate Dehydrogenase Assay, Real-time Polymerase Chain Reaction, Control